Transfection is widely used to explore gene function, analyse cellular pathways, and model disease mechanisms in vitro. In human iPSC-derived cells, it allows scientists to introduce genes or reporters directly into relevant cell types, without the need to generate stable lines.

However, achieving reliable transfection in post-mitotic, fully differentiated cells requires adapted strategies. Standard methods optimised for dividing cell lines often lead to poor efficiency or compromised cell health.

In this webinar, our two experts will share a practical overview of mRNA transfection approaches tailored for human iPSC-derived cells. Using glutamatergic neurons and microglia as examples, the session will cover optimised workflows and actionable tips that deliver robust transgene expression.

Key learnings

• Relevance of transfection in research on human iPSC-derived cells
• Key considerations when transfecting terminally differentiated cells
• Advantages of mRNA transfection for non-dividing cells compared with plasmid or viral delivery
• Step-by-step guidance through optimised protocols for ioGlutamatergic Neurons and ioMicroglia
• Recommended reagents, timing, and handling steps to maintain cell health and experimental reproducibility
• Practical approaches to evaluate transfection efficiency and expression outcomes
• Troubleshooting guidance for low efficiency or signs of cytotoxicity

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