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, well before the real search-insights.js * so the download * begins as early as before. Repeat calls share the same downloads, a * failed load is retried on the next call, and the versions / SRI * hashes live only here. * * Algolia Insights is unaffected: window.aa above is still created in * on every page and queues events until search-insights loads. * * window.loadAlgoliaLibs({ priority: 'high' }) (Oct 2026): used by the * /search page, where the first results wait on these libraries. Chrome * gives async scripts low priority, behind images and fonts; this asks * for high instead (fetchpriority). Only the first call for a library * sets its priority - later calls share that download. */ (function (win, doc) { var LIBS = [ { ready: function () { return !!win['algoliasearch/lite']; }, src: 'https://cdn.jsdelivr.net/npm/algoliasearch@5.52.1/dist/lite/builds/browser.umd.js', integrity: 'sha256-I2oYUIvhx1g6l4lTP+yoERbzLIHKzw4AKaBRQPwIeDY=' }, { ready: function () { return !!win.instantsearch; }, src: 'https://cdn.jsdelivr.net/npm/instantsearch.js@4.98.0/dist/instantsearch.production.min.js', integrity: 'sha256-ngmqZk4WBxUw257aMYZayyNBd983INngagv0jI6Ecfs=' } ]; function loadOne(lib, options) { if (lib.ready()) return Promise.resolve(); // One download per library, shared by every caller; reset on // failure so the next call retries just the library that failed. if (lib.pending) return lib.pending; lib.pending = new Promise(function (resolve, reject) { var script = doc.createElement('script'); script.src = lib.src; script.integrity = lib.integrity; script.crossOrigin = 'anonymous'; script.async = true; if (options && options.priority === 'high') script.setAttribute('fetchpriority', 'high'); script.onload = function () { lib.ready() ? resolve() : reject(new Error('Loaded but not available: ' + lib.src)); }; script.onerror = function () { script.remove(); reject(new Error('Could not load ' + lib.src)); }; doc.head.appendChild(script); }); lib.pending.catch(function () { lib.pending = null; }); return lib.pending; } win.loadAlgoliaLibs = function (options) { return Promise.all(LIBS.map(function (lib) { return loadOne(lib, options); })); }; })(window, document); . */ window.hsConversationsSettings = window.hsConversationsSettings || {}; window.hsConversationsSettings.loadImmediately = false; (function (win) { var EVENTS = ['pointerdown', 'keydown', 'touchstart', 'scroll', 'mousemove', 'wheel']; var done = false; /* 19 Oct 2026 - chat widget layout shift (field CLS). Phones fail Core Web Vitals on CLS (p75 ~0.2 site-wide in CrUX for months). Biggest cause found: HubSpot's chat container is fixed to the bottom-right corner and changes size - 100x96 launcher to 282x253 when the welcome message pops up (~5 s after load), and to full screen when a visitor opens the chat. Its top-left corner moves, so Chrome scores it as a layout shift: 0.088 per page view at 360x640 (0.044 at 412x850), on every page. Taps happen inside the chat iframe, so the parent page never sees the input and the open/close shifts count too. Fix: whenever the container's size changes it is made transparent for 400 ms (Chrome doesn't score shifts of invisible elements), then shown again at its new size. Tested on live io1001 at 360x640 in a cookie-less frame: the 0.088 shift no longer appears. The chat itself is unchanged - it just appears 0.4 s later at each resize. Relies on HubSpot's container id; if that changes this does nothing (no error). */ function cloakChatResizes() { var ID = 'hubspot-messages-iframe-container'; function watch(c) { if (c.getAttribute('data-bb-cloak')) return; c.setAttribute('data-bb-cloak', '1'); var last = c.offsetWidth + 'x' + c.offsetHeight, timer = null; new MutationObserver(function () { var size = c.offsetWidth + 'x' + c.offsetHeight; if (size === last) return; last = size; c.style.setProperty('opacity', '0', 'important'); clearTimeout(timer); timer = setTimeout(function () { c.style.removeProperty('opacity'); }, 400); }).observe(c, { attributes: true, attributeFilter: ['style', 'class'], subtree: true, childList: true }); } var found = document.getElementById(ID); if (found) { watch(found); return; } if (!document.body || !('MutationObserver' in win)) return; var mo = new MutationObserver(function () { var c = document.getElementById(ID); if (c) { mo.disconnect(); watch(c); } }); mo.observe(document.body, { childList: true }); setTimeout(function () { mo.disconnect(); }, 30000); } function loadWidget() { var api = win.HubSpotConversations; if (api && api.widget && api.widget.status && api.widget.status().loaded) return; try { cloakChatResizes(); } catch (e) {} if (api && api.widget) api.widget.load(); } function onFirstInteraction() { if (done) return; done = true; EVENTS.forEach(function (type) { win.removeEventListener(type, onFirstInteraction, { passive: true }); }); var start = function () { if (win.HubSpotConversations) loadWidget(); else (win.hsConversationsOnReady = win.hsConversationsOnReady || []).push(loadWidget); }; if ('requestIdleCallback' in win) win.requestIdleCallback(start, { timeout: 1500 }); else setTimeout(start, 200); } EVENTS.forEach(function (type) { win.addEventListener(type, onFirstInteraction, { passive: true }); }); })(window);

91探花

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GFP ioMotor Neurons
91探花_GFP_ioMotorNeurons_HERO_GFP_D11
91探花_GFP_ioMotorNeurons_FACS_Analysis_GFP_WT_vs_GFP_D1_D11_D21
91探花_GFP_ioMotorNeurons_IF_Panel_D11_DAPI_GFP_CHAT_ISL2-1
91探花_GFP_ioMotorNeurons_IF_Panel_D11_DAPI_MAP2_VACHT_HB9-1
91探花_GFP_ioMotorNeurons_vs_WT_Brightfield_D1_D4_D11_D14
91探花_GFP_ioMotorNeurons_vs_WT_RTqPCR_OCT4_TUBB3_MAP2_CHAT_VACHT_HB9_ISL2
Human iPSC-derived skeletal myocytes and motor neurons in co-culture.
91探花_GFP_ioMotorNeurons_HERO_GFP_D11
91探花_GFP_ioMotorNeurons_FACS_Analysis_GFP_WT_vs_GFP_D1_D11_D21
91探花_GFP_ioMotorNeurons_IF_Panel_D11_DAPI_GFP_CHAT_ISL2-1
91探花_GFP_ioMotorNeurons_IF_Panel_D11_DAPI_MAP2_VACHT_HB9-1
91探花_GFP_ioMotorNeurons_vs_WT_Brightfield_D1_D4_D11_D14
91探花_GFP_ioMotorNeurons_vs_WT_RTqPCR_OCT4_TUBB3_MAP2_CHAT_VACHT_HB9_ISL2
Human iPSC-derived skeletal myocytes and motor neurons in co-culture.

cat no | io1112 Early Access

GFP ioMotor Neurons

Human iPSC-derived motor neurons constitutively expressing GFP

  • Cryopreserved human iPSC-derived cells powered by opti-ox that are ready for experiments in days
  • Motor Neurons constitutively expressing GFP, easy to track in multi-cell cultures, ideal for live-cell imaging
  • Co-culture compatible with ioSkeletal Myocytes and astrocytes

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91探花_GFP_ioMotorNeurons_HERO_GFP_D11

Human iPSC-derived motor neurons constitutively expressing GFP

91探花_GFP_ioMotorNeurons_FACS_Analysis_GFP_WT_vs_GFP_D1_D11_D21

Flow cytometry analysis of GFP expression at day 1, day 11 and day 21

Flow cytometry analysis demonstrating GFP expression in >99% of cells for GFP ioMotor Neurons at Day 1 (centre-left) and Day 11 (centre-right). No GFP expression seen in wild-type ioMotor Neurons (left). At day 21, the percentage of cells expressing GFP has not decreased, indicating there is no silencing of the reporter gene (right). 

91探花_GFP_ioMotorNeurons_IF_Panel_D11_DAPI_GFP_CHAT_ISL2-1

GFP ioMotor Neurons exhibit comparable expression of neuron-specific markers to the wild-type control

Immunofluorescent staining 11 days post-revival shows similar homogenous expression of cholinergic marker (ChAT), and motor neuron marker (ISL2) in both GFP ioMotor Neurons and the wild-type control.

The GFP signal is visible exclusively in GFP ioMotor Neurons and absent in the wild-type control.

91探花_GFP_ioMotorNeurons_IF_Panel_D11_DAPI_MAP2_VACHT_HB9-1

GFP ioMotor Neurons exhibit comparable expression of neuron-specific markers to the wild-type control

Immunofluorescent staining 11 days post-revival shows similar homogenous expression of pan-neuronal marker (MAP2), cholinergic marker (VAChT), and motor neuron marker (HB9) in both GFP ioMotor Neurons and the wild-type control.

91探花_GFP_ioMotorNeurons_vs_WT_Brightfield_D1_D4_D11_D14

GFP ioMotor Neurons form a homogenous neuronal network by day 4, without clumping

GFP ioMotor Neurons mature rapidly, show motor neuron morphology and form structural neuronal networks over 14 days with no sign of clumping of coalescing of cell bodies. Day 1 to 14 post thawing. 400 碌m scale bar. 

91探花_GFP_ioMotorNeurons_vs_WT_RTqPCR_OCT4_TUBB3_MAP2_CHAT_VACHT_HB9_ISL2

GFP ioMotor Neurons demonstrate gene expression of neuronal-specific and motor-neuron-specific markers following deterministic programming

Gene expression analysis demonstrates that GFP ioMotor Neurons (GFP) and wild-type ioMotor Neurons (WT) lack the expression of pluripotency marker OCT4 (POU5F1) at day 11, while robustly expressing pan-neuronal (TUBB3 and MAP2), motor-neuron-specific (HB9 and ISL2) markers, as well as cholinergic markers (CHAT & VACHT).

Gene expression levels were assessed by RT-qPCR, data normalised to HMBS; cDNA samples of the parental human iPSC line (iPSC) were included as reference. Data represents day 11 post-revival samples.

View the step-by-step RNA extraction and RT-qPCR protocol used to generate this data

Human iPSC-derived skeletal myocytes and motor neurons in co-culture.

Co-culture of ioMotor Neurons and ioSkeletal Myocytes

High resolution confocal imaging of ioSkeletal Myocytes (io1002) and ioMotor Neurons wild-type co-culture. Staining with alpha-bungarotoxin (yellow) highlights acetylcholine receptor expression on co-cultured ioSkeletal Myocytes. Desmin (cyan) and microtubule-associated protein 2 (red) define ioSkeletal Myocytes and ioMotor Neurons respectively. Co-culture imaged at day 30, 40X magnification.

Download the step-by-step protocol for culturing ioSkeletal Myocytes and ioMotor Neurons.

Vial limit exceeded

A maximum number of 20 vials applies. If you would like to order more than 20 vials, please contact us at orders@91探花.

Human iPSC-derived motor neurons constitutively expressing GFP

GFP ioMotorNeurons are a fluorescent human lower motor neuronal model derived from our well-established wild-type ioMotor Neurons. These cells are engineered to constitutively express green fluorescent protein (GFP), and provide a powerful, ready-to-use tool for diverse applications.

Stable GFP expression enables easy, real-time tracking in complex, multi-cellular systems, facilitating the study of cellular interactions with glia, or neuro-muscular interactions when cultured with our ioSkeletal Myocytes. The cells are ideal for live cell imaging for assessment of neurite  outgrowth, neuronal morphology and survival in response to compound treatment.

GFP ioMotor Neurons are delivered cryopreserved and ready-to-culture. This eliminates the time and effort required to engineer your own GFP-expressing iPSC lines and manage complex directed differentiation protocols.

Benchtop benefits

quick_13

Live-cell imaging ready

Assess neurite outgrowth, neuronal morphology and survival in real-time.

culture_9

Co-culture compatible

Easily track GFP motor neurons in complex cultures with glia or skeletal myocytes.

easy_13

Easy-to-use

Within 4 days post revival cells are ready for experimentation, displaying motor neuronal morphology, without clumping.

Cells arrive ready to plate


91探花_ioMotorNeurons_user_manual_timelines_D11+

GFP ioMotor Neurons are delivered in a cryopreserved format and are programmed to rapidly mature upon revival in the recommended media.

Product specifications

Download product brochure

Starting material

Human iPSC line

Seeding compatibility

6, 12, 24, 96 & 384 well plates

Shipping info

Dry ice

Donor

Caucasian adult male, age 55-60 years old (skin fibroblast),
Genotype APOE 3/4

Vial size

Small: >1 x 10鈦� viable cells

Quality control

Sterility, protein expression (ICC), gene expression (RT-qPCR) and GFP expression (flow cytometry)

Differentiation method

opti-ox deterministic cell programming

Recommended minimum seeding density

30,000 cells/cm虏

User storage

LN2 or -150掳C

Format

Cryopreserved cells

Product use

ioCells are for research use only

Applications

Live-cell imaging
Co-culture
High-content screening
Neurotoxicology
Drug discovery

 

Scale your study with volume pricing

Enabling scientists to use human cells in their research, running additional experiments without rationing cells or limiting experimental scale

 

Order quantity Total vials received Pricing tier 
1 - 9 packs 3 - 27 vials Standard price
10 - 33 packs 30 - 99 vials Automatic 10% discount
> 34 packs > 100 vials > Contact us for a quote

 

Academic pricing: Academic users can purchase any ioCells in 3-vial packs ($/鈧�/拢 999 per pack), available year-round with any cell type combination.

ioMotor Neurons Customer Testimonials

An image of Dr Irit Reichenstein

Dr Irit Reichenstein

Senior Scientist | Anima Biotech

"We did use the cells and were very happy with them :) They were very homogenous (unlike motor neurons from other vendors that we worked with), viable and absolutely beautiful. We got wonderful results with them."

An image of Dr Elizabeth Di Lullo

Dr Elizabeth Di Lullo

Associate Scientific Director | Brainever

鈥淲e thawed the cells and they looked wonderful. Close to 100% viable and looked great in cell culture! No clumping and easy to culture.鈥�
Technical data

Highly characterised and defined

Flow cytometry analysis of GFP expression at day 1, day 11 and day 21

91探花_GFP_ioMotorNeurons_FACS_Analysis_GFP_WT_vs_GFP_D1_D11_D21

Flow cytometry analysis demonstrating GFP expression in >99% of cells for GFP ioMotor Neurons at Day 1 (centre-left) and Day 11 (centre-right). No GFP expression seen in wild-type ioMotor Neurons (left). At day 21, the percentage of cells expressing GFP has not decreased, indicating there is no silencing of the reporter gene (right).

GFP ioMotor Neurons exhibit comparable expression of neuron-specific markers to the wild-type control

91探花_GFP_ioMotorNeurons_IF_Panel_D11_DAPI_GFP_CHAT_ISL2-1
91探花_GFP_ioMotorNeurons_IF_Panel_D11_DAPI_MAP2_VACHT_HB9-1

Immunofluorescent staining 11 days post-revival shows similar homogenous expression of pan-neuronal markers (MAP2 & TUBB3), cholinergic markers (ChAT & VAChT), and motor neuron markers (HB9 & ISL2) in both GFP ioMotor Neurons and the wild-type control.

The GFP signal is visible exclusively in GFP ioMotor Neurons and absent in the wild-type control.

View the step-by-step immunofluorescent staining protocol used to generate this data 

GFP ioMotor Neurons form a homogenous neuronal network by day 4, without clumping

91探花_GFP_ioMotorNeurons_vs_WT_Brightfield_D1_D4_D11_D14

GFP ioMotor Neurons mature rapidly, show motor neuron morphology and form structural neuronal networks over 14 days with no sign of clumping of coalescing of cell bodies. Day 1 to 14 post thawing. 400 碌m scale bar.

GFP ioMotor Neurons demonstrate gene expression of neuronal-specific and motor-neuron-specific markers following deterministic programming

91探花_GFP_ioMotorNeurons_vs_WT_RTqPCR_OCT4_TUBB3_MAP2_CHAT_VACHT_HB9_ISL2

Gene expression analysis demonstrates that GFP ioMotor Neurons (GFP) and wild-type ioMotor Neurons (WT) lack the expression of pluripotency marker OCT4 (POU5F1) at day 11, while robustly expressing pan-neuronal (TUBB3 and MAP2), motor-neuron-specific (HB9 and ISL2) markers, as well as cholinergic markers (CHAT & VACHT).

Gene expression levels were assessed by RT-qPCR, data normalised to HMBS; cDNA samples of the parental human iPSC line (iPSC) were included as reference. Data represents day 11 post-revival samples.

View the step-by-step RNA extraction and RT-qPCR protocol used to generate this data

GFP expression

Flow cytometry analysis of GFP expression at day 1, day 11 and day 21

Flow cytometry analysis demonstrating GFP expression in >99% of cells for GFP ioMotor Neurons

Flow cytometry analysis demonstrating GFP expression in >99% of cells for GFP ioMotor Neurons at Day 1 (centre-left) and Day 11 (centre-right). No GFP expression seen in wild-type ioMotor Neurons (left). At day 21, the percentage of cells expressing GFP has not decreased, indicating there is no silencing of the reporter gene (right).

Co-culture with ioSkeletal Myocytes

Co-culture of ioMotor Neurons and ioSkeletal Myocytes

Human iPSC-derived skeletal myocytes and motor neurons in co-culture.

High resolution confocal imaging of ioSkeletal Myocytes (io1002) and ioMotor Neurons wild-type co-culture. Staining with alpha-bungarotoxin (yellow) highlights acetylcholine receptor expression on co-cultured ioSkeletal Myocytes. Desmin (cyan) and microtubule-associated protein 2 (red) define ioSkeletal Myocytes and ioMotor Neurons respectively. Co-culture imaged at day 30, 40X magnification.

Download the co-culture protocol

How to culture ioMotor Neurons

In this video, our scientist will take you through the step-by-step process of how to thaw, seed and culture ioMotor Neurons.

Frequently Asked Questions (FAQs)

Is the GFP signal in GFP ioMotor Neurons expressed in a specific subcellular region?

GFP ioMotor Neurons consist of eGFP expression under the control of a constitutive promoter and is expected to be distributed across the cell.

 

 

Do GFP ioMotor Neurons retain key characteristics and functionality of ioMotor Neurons wild-type?

GFP ioMotor Neurons exhibit comparable marker and gene expression to the genetically-matched wild-type control ioMotor Neurons. GFP ioMotor Neurons have been designed to enable easy tracking in multi-cell cultures, being ideal for multi-cell cultures.

 

Expand your research

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Build ALS-FTD disease models in vitro
Model ALS and FTD with human iPSC-derived neurons
Expand your research
Build ALS-FTD disease models in vitro
Model ALS and FTD with human iPSC-derived neurons
Modeling-disease-with-isogenic-control

Access 14 ALS and FTD disease models with disease-related mutations such as SOD1, FUS, MAPT and TDP-43 (TARDBP) genetically engineered in ioGlutamatergic Neurons and ioMotor Neurons.

Find out more

 

CRISPR-ready hiPSC-derived cells ready for CRISPR screens
Simplify gene knockouts
Use CRISPRko-Ready ioMotor Neurons cells to study your gene of interest
Expand your research
Simplify gene knockouts
Use CRISPRko-Ready ioMotor Neurons cells to study your gene of interest
CRISPR-ready hiPSC-derived cells ready for CRISPR screens

Interested in gene knockouts and CRISPR screens? 
CRISPRko-Ready ioMotor Neurons cells are engineered to constitutively express Cas9 nuclease for the quick and easy generation of gene knockouts and CRISPR screens.

Explore CRISPRko-Ready ioMotor Neurons.

Co-culture-hiPSC-derived-motor-neurons-and-astrocytes
Study ALS in complex cultures
Co-culture motor neurons with astrocytes to gain insights into electrical activity
Expand your research
Study ALS in complex cultures
Co-culture motor neurons with astrocytes to gain insights into electrical activity
Co-culture-hiPSC-derived-motor-neurons-and-astrocytes

Study neuronal networks and the impact of ALS-disease-related mutations by co-culturing ioMotor Neurons with astrocytes. Access 14 disease models and the single co-culture protocol for MEA.

View the co-culture protocol

Explore ALS & FTD Disease Models

Explore ioAstrocytes

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Custom cell development
Generate custom disease models or reporter lines
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Custom cell development
Generate custom disease models or reporter lines
Engineer-disease-mutation-hiPSC-derived-cells

Build your custom disease model or reporter line to pair with wild-type ioMotor Neurons as the genetically matched control.

Throughout the custom process, our experts will bring your project to life, and be on hand to support you with any technical queries.

Start the conversation today 

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